ABSTRACT
Lactobacilli are the well known friendly bacteria for their probiotic activities against pathogens. The inhibitory activity of different strains of lactobacilli either obtained as commercial products or isolated from human feces was investigated against the clinical isolates of Pseudomonas aeruginosa. The isolates were selected as the most resistant strains when challenged with anti-pseudomonal antibiotics already in clinical practice. Both the plate spot test as well as the agar cup method were used for screening of Lactobacillus strains against Pseudomonas aeruginosa. A Lactobacillus acidophilus strain isolated from feces of an Iranian child showed a strong anti-pseudomonal activity [90 percent after 72h incubation] against the multi-drug resistant clinical isolates while a Lactobacillus reuteri strain isolated from a commercial oral product resulted in relatively weak response and a Lactobacillus acidophilus strain isolated from a commercial vaginal product did not show any inhibitory activity. In a kinetic study the lactobacillus sensitive Pseudomonas aeruginosa showed a significant bacteriostatic activity in vitro in the presence of lactobacillus supernatants. Some lactobacilli exhibit significant inhibitory activity against the multidrug resistant clinical isolates of Pseudomonas aeruginosa
ABSTRACT
Geum kokanicum Regel et schmath, a member of Rosaceae family, is an endemic perennial rhizomatous plant of Iran. This plant has a consideration attention in the treatment of diarrhea and other gastrointestinal disorders. In this study, the anti bacterial effects of total extract and fractions of rhizome of plant were investigated. Rhizomes of G. Kokanicum were collected in June 2006 from Bojnourd [khorasan province]. The dried rhizomes were extracted with ethanol to prepare the total extract. Another part of the rhizomes was separately extracted with petrolium ether, dichloromethane, methanol and water successively in order to obtain different fractions. The antibacterial effects of total extract and fractions were investigated with disc diffusion method. The results shows Staphylococcus aureus were susceptible against total extract and methanol fraction. This fraction [10mg/disc] was indicated the largest zone diameters [19mm]. The MIC Values of total extract was investigated. Among the bacterial strains, Staphylococcus epidermitus demonstrated high sensitivity to the total extract of G. kokanicum [0.125mg/ml]. The zone diameters of total extract was 18 mm on S. aureus in 10 mg/disc. Phytochemical analysis of total extract was shown the large amounts of tanan compounds. We suspect the antibacterial effect is for tanan compounds. This was demonstrated by bioautography method analysis of tanan part isolated form methanol fraction
Subject(s)
Anti-Infective Agents , Plant Extracts , Rhizome , EthanolABSTRACT
Rising challenges in the present drug discovery model are prompting Prompt a renewed interest in natural products as a source of novel, bioactive small molecules. With the current validation of zebra fish as a biomedical appropriate model for functional genomics and in vivo drug discovery, the zebra fish bioassay-guided identification of natural products may be an attractive tactic to generate new lead compounds in a number of indication regions. Here, we review recent natural product research using zebra fish and evaluate the potential of this vertebrate model as a discovery platform for the systematic identification of bioactive natural products
Subject(s)
Animals , Biological ProductsABSTRACT
From the hexane extract of the aerial parts of Ferulago Bernardii [Apiaceae] four coumarins, namely prantschimgin 1, oxypeucedanin 2, psoralen 3 and umbelliferone 4; beta-sitosterol 5; and nonacosane 6 were isolated by Column Chromatography [CC], Preparative Thin Layer Chromatography [PTLC] and crystallization. The structures were elucidated by melting point, UV, IR, MS, 1H and 13C-NMR spectra. The presence of compounds 1, 2, 3 and 5 in some others Ferulago species could be used as chemotaxonomic marker in genus Ferulago. This is the first report on phytochemical analysis of Ferulago Bernardii Tomk. and M. Pimen